I-YYT42 - I-Aerosols Aerosols Penettation Vietation
Bheka kulezi zibalo ezilandelayo ngenkathi ufunda lesi sahluko.
Amazinga
I-ISO / i-Dis 22611 Izingubo zokuvikelwa ekuvikelekeni ekulweni nezezimali ze-Agents-Test zokumelana nokungena nge-aerosols engcolisiwe.
Ukucacisa
I-Aerosol Generator: Unsomi
Igumbi lokuvezwa:I-pmma
Umhlangano wesampula:2, insimbi engagqwali
Iphampu ye-vacamm:Kufika ku-80kpa
Ubukhulu: I-300mm * 300mm * 300mm
Ukunikezwa kwamandla kagesi:220v 50-60hz
Ubukhulu bemishini: 46cm × 93cm × 49cm (H)
Isisindo se-Net: 35kg
Amalungiselelo
Beka lezi zingxenye ezintathu kwikhabethe le-biosafety. Hlola izingxenye ngazinye zomshini wokuhlola futhi uqiniseke ukuthi zonke izingxenye ezisebenza kahle futhi zixhuma kahle.
Ukusika amasampula ayisishiyagalombili njengemibuthano yobubanzi engama-25mm.
Lungiselela isiko ubusuku bonke we-staphylococcus aureus ngokudluliselwa kwe-asuptic kwe-bacterium kusuka ku-agar yezakhi (okugcinwe ngo-4 ± 1 ℃) kumhluzi wezakhi kanye nokufakwa ku-37 ± ku-orbital shaker.
Dlulisa isiko libe yivolumu efanelekile ye-sterile isotonic saline ukunikeza ukubalwa kwamagciwane okugcina kwe-5 * 107Amaseli cm-3usebenzisa igumbi lokubala le-Thoma.
Gcwalisa isiko elingenhla ku-atomizer. Ileveli ye-Liquid iphakathi kwezinga eliphakeme neleveli ephansi.
Ukuhlinza
Faka umhlangano wesampula. Beka i-silicone washer a, indwangu yokuhlola, i-silicone washer b, membrane, ukusekelwa kocingo kwisembozo esivulekile, ikhava ngesisekelo.
Faka omunye umhlangano wesampula ngaphandle kwesampula.
Vula isembozo esingaphezulu segumbi lokuhlola.
Faka umhlangano wesampula nesampula nomhlangano ngaphandle kwesampula ngokubopha umdwebo we-Fig. 4-1.
Qiniseka ukuthi wonke amashubhu axhunyiwe kahle.
Xhuma umoya ocindezelwe ekuguqukeni komoya okucindezelwe.
Faka umoya ngokugeleza kwe-5l / imizuzu ngokulungisa imitha yokugeleza ku-atomizer bese uqala ukukhiqiza i-aerosol.
Ngemuva kwamaminithi ama-3 asebenze i-vaccing pump. Setha njenge-70kpa.
Ngemuva kwamaminithi ama-3, cisha umoya ku-atomizer, kepha shiya ipompo ye-vacuum isebenza nge-1 min.
Cisha iphampu ye-VUMUM.
Susa imihlangano yesampula egumbini. Futhi ngokudlulisela ulwelwesi lwe-0.45um kumabhodlela wendawo yonke aqukethe i-10ml sterile isotonic saline.
Khipha ngokuxhawula imizuzu eyi-1. Futhi wenze ama-serial diartion ngosawoti oyinyumba. (10-1, 10-2, 10-3, no-10-4)
Plate ngaphandle kwe-1ml aliquots ye-dilution ngayinye ephindwe kabili usebenzisa i-agar yezakhi.
Ukufaka amapuleti ubusuku bonke ku-37 ± 1 ± futhi uveze imiphumela usebenzisa isilinganiso sokubala kwangemuva kwe-bacterial kunombolo yeBactria edlula i-Throg isampula yokuhlola.
Yenza izinqumo ezine kuhlobo ngalunye lwendwangu noma isimo sendwangu.
Njengayo yonke imishini kagesi, le yunithi kufanele isetshenziswe kahle futhi ukunakekelwa kanye nokuhlola kumele kwenziwe ngezikhathi ezithile. Ukuqapha okunjalo kuzoqinisekisa ukusebenza okuphephile nokusebenzayo kwemishini.
Ukulungiswa ngezikhathi ezithile kuqukethe ukuhlolwa okwenziwe ngokuqondile ngu-opharetha wokuhlola kanye / noma ngabasebenzi abagunyaziwe abagunyaziwe.
Ukulungiswa kwemishini kungumsebenzi womthengi futhi kufanele kwenziwe njengoba kushiwo yilesi sahluko.
Ukwehluleka ukwenza izenzo zokugcina ezenziwayo noma ukunakekelwa okwenziwe ngabantu abangagunyaziwe kungakwenza iwaranti.
1. Umshini kumele uhlolwe ukuvikela ukuvuvukala kokuxhumeka ngaphambi kokuhlolwa;
2. Ukuhambisa umshini kwenqatshelwe lapho kuyisebenzisa;
3. Khetha ukunikezwa kwamandla okuhambisanayo kanye namandla kagesi. Ungaphezulu kakhulu ukugwema idivaysi evuthayo;
4. Sicela uxhumane nathi ukuze usingathe ngesikhathi lapho umshini ungaphandle kwe-oda;
5. Kumele kube nendawo yokupholisa umoya omncane lapho umshini usebenza;
6. Ukuhlanza umshini ngemuva kokuhlolwa ngaso sonke isikhathi;
| Ukwenza | I-WHO | Nini |
| Hlola ukuqinisekisa ukuthi alukho umonakalo wangaphandle emshinini, olungakwazi ukubeka engcupheni ukuphepha kokusetshenziswa. | Osebenzayo | Ngaphambi kwazo zonke izikhathi zokusebenza |
| Ukuhlanza umshini | Osebenzayo | Ekugcineni kokuhlolwa ngakunye |
| Ihlola ukuvuza kokuxhuma | Osebenzayo | Ngaphambi kokuhlolwa |
| Ukuhlola isimo nokusebenza kwezinkinobho, umyalo we-opharetha. | Osebenzayo | -Masonto onke |
| Ukuhlola intambo yamandla kunamathiselwe kahle noma cha. | Osebenzayo | Ngaphambi kokuhlolwa |
